What is the laboratory technique called that amplifies DNA in vitro by incubating with primers, DNA polymerase, and nucleotides?

What is the laboratory technique called that amplifies DNA in vitro by incubating with primers, DNA polymerase, and nucleotides?

• A. Restriction enzymes
• B. PCR
• C. DNA ligase
• D. Transcription

Answer: (B)

Amplifying DNA in vitro using primers, DNA polymerase, and nucleotides is Polymerase Chain Reaction. In PCR, the DNA is repeatedly heated to separate strands, cooled to allow primers to bind to their matching sequences, and heated again for a DNA polymerase to extend new strands using the supplied nucleotides. Each cycle increases the amount of target DNA, producing exponential amplification. Restriction enzymes cut DNA at specific sequences, rather than amplifying it. DNA ligase seals breaks to join DNA fragments. Transcription is the process of making RNA from a DNA template.

Amplifying DNA in vitro using primers, DNA polymerase, and nucleotides is Polymerase Chain Reaction. In PCR, the DNA is repeatedly heated to separate strands, cooled to allow primers to bind to their matching sequences, and heated again for a DNA polymerase to extend new strands using the supplied nucleotides. Each cycle increases the amount of target DNA, producing exponential amplification.

Restriction enzymes cut DNA at specific sequences, rather than amplifying it. DNA ligase seals breaks to join DNA fragments. Transcription is the process of making RNA from a DNA template.