An immobilized enzyme is characterized by which statement?

An immobilized enzyme is characterized by which statement?

• A. Enzymes fixed to a solid support and cannot be reused.
• B. Enzymes fixed to a solid support and can be reused across multiple reaction cycles.
• C. Enzymes that operate only in free solution and cannot be immobilized.
• D. Enzymes that are only active at extreme pH levels.

Answer: (B)

Immobilized enzymes are enzymes attached to a solid support or trapped in a matrix, so they stay in place while the reaction occurs and can be separated from the product. This setup lets the enzyme be reused across multiple reaction cycles, which is a central advantage in many applications and a defining feature of immobilization. The option that describes enzymes fixed to a solid support and usable for several rounds captures this reuse and persistence. In contrast, saying the enzyme cannot be reused, or that enzymes must operate only in free solution and cannot be immobilized, describes scenarios that do not reflect immobilization. The idea that they are active only at extreme pH levels is unrelated to immobilization and does not describe the concept.

Immobilized enzymes are enzymes attached to a solid support or trapped in a matrix, so they stay in place while the reaction occurs and can be separated from the product. This setup lets the enzyme be reused across multiple reaction cycles, which is a central advantage in many applications and a defining feature of immobilization. The option that describes enzymes fixed to a solid support and usable for several rounds captures this reuse and persistence. In contrast, saying the enzyme cannot be reused, or that enzymes must operate only in free solution and cannot be immobilized, describes scenarios that do not reflect immobilization. The idea that they are active only at extreme pH levels is unrelated to immobilization and does not describe the concept.